Custom Chicken Antibody Production
Abcore is an antibody production company located in Southern California and we are focused on making high quality antibodies. Our emphasis is on animal health, and our chickens bred and put into production on the same farm. Abcore also has on site veterinarians which monitor chicken health and comfort to maximize egg production.
Project Information for Custom Chicken Antibodies
Abcore offers standard 56 day chicken production packages which consist of 4 immunizations, resulting in the collection of 12-15 eggs per chicken (please see example project timeline below). We typically use 2 chickens for each project, and prices start at $330. Our chickens are California White Leghorn and were developed specifically for poultry operations in California.
Abcore provides free antigen design to assist in selection of the best possible peptide sequence for eliciting the desired antibody response. We use the latest in software with the proper algorithms to assess antigenicity, hydrophilicity, flexibility, secondary structure and aggregation potential. Please take advantage of this free assistance.
Standard 56 day, 2 Chicken Antibody Production Protocol
| Week | Action |
|---|---|
| Pre-immunization egg collection | |
| 0 | Immunize chickens with 0.25 mg of antigen mixed with Complete Freund's Adjuvant |
| 2 | Immunize chickens with 0.15 mg of antigen mixed with Incomplete Freund's Adjuvant |
| 4 | Immunize chickens with 0.15 mg of antigen mixed with Incomplete Freund's Adjuvant |
| 5 | Start egg collection |
| 7 | Immunize chickens with 0.15 mg of antigen mixed with Incomplete Freund's Adjuvant |
| 8 | End egg collection |
Please note:
- Antigen requirement for custom chicken antibody production: 1.4 mg per 2 chicken protocol for immunizations.
- Yield is typically 12-15 eggs per chicken.
- IgY yield is approximately 50 mg/yolk.
- Yolk IgY isolation is available for a nominal cost, and can be shipped in PBS containing 0.1% NaN3. We can also ship intact eggs.
Additional Optional Services:
- Additional egg collection
- Additional immunizations
- Additional ELISA assays
- IgY purification
- Affinity purification
- Western blot validation
- IHC validation
- ICC validation
Please contact us for detailed pricing information or with any questions you may have by calling 1-888-359-1364 or emailing us at info@abcore-inc.com
Order forms and additonal information
Polyclonal Antibody Production Order Form (PDF)
Peptide Synthesis Order Form (PDF)
Custom Antibody Production Information for Europe (PDF)
Custom Antibody Production Information for the United States (PDF)
Abcore USDA registration number 93-R-0541.