baculoQUANT™, is a novel one-step virus titration kit which can give an accurate result within 2 hours without the need for plaque assays or immunoassays.
baculoQUANT™ is a fast and cost-effective method of recombinant virus titration. It is based on a technique called quantitative PCR (QPCR). QPCR utilizes a set of primers and a probe which are designed to specifically target and amplify a small region of the baculovirus genome. The increase in amplification of this small region is then correlated to virus genome copy number and can be used to measure the number of recombinant virus particles present in an infected insect cell culture. This novel titration method has been proven to be a very accurate, reproducible and fast way to titrate recombinant baculoviruses, both for single-use titrations and for high throughput applications on automated systems.
Compared with other methods of virus titration such as plaque assay, end-point dilution and ELISA, which require anywhere from 24 to 120 hours to yield a result and involve specialised procedures,baculoQUANT™ can provide a result within 2 hours, dramatically improving the productivity of the protein production process.
For more information and protocol details, please download the baculoQUANT™ Userguide (PDF).