Anti-CD18 Antibody (Pacific Blue™)

$315.00
Product Number: PB-216

Anti-CD18 monoclonal antibody raised in mouse, conjugated to Pacific Blue™.

Antibody Information
Host Species: 
Mouse
Clonality: 
Monoclonal (MEM-48)
Clone: 
MEM-48
Isotype: 
IgG1
Conjugate: 
Pacific Blue™
Immunogen Type: 
Anti-CD18 antibody (Pacific Blue™) was raised against leukocytes of a patient suffering from a LGL-type leukemia.
Species Reactivity: 
Human
Negative Species Reactivity: 
Canine (Dog)
Specificity: 

Anti-CD18 antibody (Pacific Blue™) recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Product Information
Amount: 
100 tests
Buffer: 
Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free bovine serum albumin (BSA) as a stabilizing agent.
Storage: 
Store antibody in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Short-term exposure to room temperature should not affect the quality of the antibody. However, if reagent is stored under any conditions other than those specified, the conditionsmust be verified by the user.
Application Details
Tested Applications: 
WB, IHC(F), IHC(P), IP, FC, FUNC
Application Overview: 
Anti-CD18 antibody (Pacific Blue™) can be used for western blot, immunohistochemistry using frozen sections, immunohistochemistry using paraffin sections, immunoprecipitation, flow cytometry, functional applications.
Additional Information
Alternative Names: 
CD18, Integrin beta2 subunit
Product Citation(s): 

Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50.

Stefanova I, Horejsi V: Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules. J Immunol. 1991 Sep 1;147(5):1587-92.

Larson RS, Springer TA: Structure and function of leukocyte integrins. Immunol Rev. 1990 Apr;114:181-217.

Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000 Jun 1;164(11):5928-34.

Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93(10):3505-11.

Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects. Blood. 1997 Oct 15;90(8):3187-94.

Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113(2):358-69.

Background Publication(s): 

Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V.: Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun. 2000 Aug 28;275(2):295-9.

Gao JX, Issekutz AC: Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating humanneutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996 Jul;88(3):463-70.

Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to humanmonocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol. 1998 Jun;28(6):1970-9.

Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J Immunol. 2003 Dec 1;171(11):5697-706.

Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the functions of integrinalphaMbeta2. J Biol Chem. 2005 Jan 14;280(2):1336-45.

Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J Investig Dermatol Symp Proc. 2006 Sep;11(1):7-15.

Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects. Infect Immun. 2007 Oct;75(10):5004-10.

Supplier Informaiton
Supplier: 
EXBIO
Supplier Product Number: 
PB-216-T100
Supplier Product Name: 
Mouse monoclonal antibody to CD18 / Integrin beta2 subunit
Antibodies
Antibodies by Application
CD Antibodies
Monoclonal Antibodies
Antibodies by Clonality
Conjugated Antibodies
Mouse Antibodies
(FC) Flow Cytometry Antibodies
CD and MHC Antibodies
Antibodies by Function
Functional Analysis Antibodies
Antibodies by Host Species
Pacific Blue™ Antibodies
(IHC) Immunohistochemistry Antibodies
(IP) Immunoprecepitation Antibodies
(WB) Western Blot Antibodies
EXBIO

New Secondary Antibodies

Abcore is pleased to announce a new complete range of secondary antibodies.

New Antibodies & Normal Serums

Abcore excited to announce the release of new antibodies and a comprehensive line of normal serums.