Alexa Fluor® 700 Antibodies

Alexa Fluor®700 antibodies are conjugated to Alexa Fluor® 700. Alexa Fluor® 700 is a molecular probe exhibiting a red color which absorbs light at 702 nm. Alexa Fluor® 700 emits light at 775 nm, and has a molar mass of approximately 1300 g/mol. Alexa Fluor® 647 has a quantum yield of .12. Molecular probes like Alexa Fluor® 700 conjugates are useful in labeling and detecting specific properties of molecules and structures. Molecular probes are attached to other molecules and can be an atom or a molecule. In the case of Alexa Fluor® 700 molecular probes, the attached molecule is a fluorescent die. This die is excitable and emits a spectrum of visible light. Alexa Fluor® 700 dyes are made by sulfonating coumarine, rhodamine, fluorescein, and cyanine. Alexa Fluor® 700 dyes are negatively charged and hydrophilic. In comparison to other common dyes, Alexa Fluor® 700 conjugated antibodies are more stable, less pH sensitive, and brighter.

CD3 antibody (Alexa Fluor®700) has been used in research regarding B cell lymphoma as well as brain tumors. The CD3 complex is a mediator of signal transduction localized on the cell membrane. Defects in CD3 and anti- CD3 antibody can cause several severe immunodeficiency disorders among a specific group of ailments characterized by low antibody levels and specific immunities due to poor T- cell development. Anti-CD105 antibody (Alexa Fluor®700) binds against a major glycoprotein of the vascular endothelium. It is localized on the membrane and expressed in endoglin restricted to endothelial cells in all tissues except bone marrow. CD105/ENG is thought to play an integral role in the binding of endothelial cells to integrins and/or RGD receptors. Defects in ENG/CD105 are the cause of Osler-Rendu-Weber Syndrome 1. Osler-Rendu-Weber Syndrome 1 is a genetic disorder in which affected individuals develop arteriovenous malformations (abnormal blood vessels) in many areas in the body. The disease is characterized by frequent nosebleeds, gastrointestinal tract bleeding, seizures, strokes, shortness of breath, and the presence of large birthmarks called “port-wine stain”.